Hofmann-Lehmann
Fakultäten » Vetsuisse-Fakultät » Nutztiere, Departement für » Veterinärmedizinisches Labor » Prof. Dr. Regina Hofmann-Lehmann » Hofmann-Lehmann
Completed research project
| Title / Titel | Development of TaqMan real-time PCR assays for feline reference genes for the investigation of gene expression levels in blood and in lymphatic, endocrine, and other feline tissues | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | Gene expression analysis is frequently used in a wide area in contemporary research, with quantitative real-time reverse transcriptase PCR as the method of choice for exact reliable and fast quantification of mRNA transcription levels. Weakness of this method is the incidence of inaccurate normalization, rendering study results questionable. One possible approach to handle this problem is the inclusion of reference genes with stable expression. As generally known, variation in gene expression of commonly used reference genes cannot be averted. It is therefore of utmost importance to analyze the expression of reference genes preliminary of their application in gene expression studies. We developed and optimized eight new candidate reference gene TaqMan real-time PCR assays (ABL, ACTB, B2M, GUSB, HMBS, HPRT, RPS7, YWHAZ) for the feline species and analyzed their suitability together with two widely used assays (GAPDH, 18S rRNA) in blood and 15 different tissues, such as lymphoid, endocrine, gastrointestinal, renal, neuronal, muscular and neoplastic tissues. This project is the basis for the doctoral thesis of Yvonne Kessler. |
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| Publications / Publikationen | Kessler Y, Helfer-Hungerbuehler AK, Cattori V, Meli ML, Zellweger B, Ossent P, Riond B, Reusch CE, Lutz H, Hofmann-Lehmann R: Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues. BMC Mol Biol 2009, 10(1):106. | ||||||
| Keywords / Suchbegriffe | quantitative real-time reverse transcriptase PCR, quantification of mRNA transcription levels, reference genes, geNorm, NormFinder, TaqMan | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
SNF (Personen- und Projektförderung) |
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| Duration of Project / Projektdauer | Jul 2007 to Dec 2009 |