Haas

Fakultäten » Medizinische Fakultät » Rechtsmedizin, Institut für » Prof. Dr. Walter Bär (emeritiert) » Haas

Completed research project

Title / Titel	mRNA profiling for low template samples / small stains
PDF	Abstract (PDF, 14 KB)
Summary / Zusammenfassung	mRNA profiling is a new method for the identification of body fluids from biological stains. Several mRNA markers were developed for the identification of blood, saliva, semen, vaginal secretions, menstrual blood and skin. The majority of stains encountered in nowadays forensic laboratories are contact stains, meaning collected cells from diverse surfaces, normally very few cells. The aim of this study is to develop mRNA profiling strategies for dealing with low template samples / small stains. One approach would be to pre-amplify the whole RNA before any down stream analysis. The developed method could then be applied to skin swabs and forensic stains.
Publications / Publikationen	Haas C, Klesser B, Maake C, Bär W, Kratzer A. mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR. Forensic Sci Int Genet. 2009 Mar;3(2):80-8
Keywords / Suchbegriffe	mRNA, body fluid identification, forensic
Project leadership and contacts / Projektleitung und Kontakte	Dr. Cordula Haas (Project Leader) cordula.haas@irm.uzh.ch Dr. Adi Kratzer krat@irm.uzh.ch Prof. Walter Bär baer@irm.uzh.ch
Funding source(s) / Unterstützt durch	No project-specific funding
In collaboration with / In Zusammenarbeit mit	Prof. J. Ballantyne United States
Duration of Project / Projektdauer	Jan 2010 to Dec 2011