Sensitive quantification of PrPSc using mass spectrometry

Completed Research Project

Title / Titel: Sensitive quantification of PrPSc using mass spectrometry

Summary / Zusammenfassung: We are developing a sensitive assay for PrPSc based on mass spectrometry and isotope coded affinity tags (ICAT). The test is designed to allow an absolute quantification of PrPSc in a sample. We are currently optimizing the discrete steps of the test protocol using infected murine brain material. Attempts are made to reproduce the detection of PrPSc in urine of prion-infected mice. If positive, adaptation of the test protocol to quantify U PrPSc in urine of diseased mice will be performed. The test will be validated as a possible assay for early diagnosis of TSEs.

Keywords / Suchbegriffe: mass spectrometry, ICAT, quantification, proteomics, PrPSc

Project Leadership and Contacts / Projektleitung und Kontakte

Erich Brunner (Project Leader)	erich.brunner@usz.ch
Nathalie Braun (Project Leader)	nathalie.braun@usz.ch
Prof. A. Aguzzi	adriano@pathol.uzh.ch

Other Links to external Webpages: http://www.uzh.ch/pathol/neuropathologie/d/index.html

Funding Source(s) / Unterstützt durch: Forschungskredit der Universität Zürich, Others
Department of Defense, USA

In Collaboration with / In Zusammenarbeit mit

Prof Dr. Ruedi Aebersold,Institute for Systemsbiology, Seattle	United States
Prof Dr. Michael Gelb, University of Washington, Seattle	United States