Brunner Braun
Fakultäten » Medizinische Fakultät » Neuropathologie, Institut für » Prof. Dr. Adriano Aguzzi » Brunner Braun
Completed research project
| Title / Titel | Sensitive quantification of PrPSc using mass spectrometry | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | We are developing a sensitive assay for PrPSc based on mass spectrometry and isotope coded affinity tags (ICAT). The test is designed to allow an absolute quantification of PrPSc in a sample. We are currently optimizing the discrete steps of the test protocol using infected murine brain material. Attempts are made to reproduce the detection of PrPSc in urine of prion-infected mice. If positive, adaptation of the test protocol to quantify U PrPSc in urine of diseased mice will be performed. The test will be validated as a possible assay for early diagnosis of TSEs. | ||||||
| Keywords / Suchbegriffe | mass spectrometry, ICAT, quantification, proteomics, PrPSc | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Other links to external web pages | http://www.uzh.ch/pathol/neuropathologie/d/index.html | ||||||
| Funding source(s) / Unterstützt durch |
Forschungskredit der Universität Zürich, Others Department of Defense, USA |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | May 2003 to Apr 2006 |