Schüpbach
Fakultäten » Medizinische Fakultät » Medizinische Virologie, Institut für » Prof. Dr. Jörg Schüpbach » Schüpbach
Completed research project
| Title / Titel | Adaptation of the quantitative HIV-1 p24 assay to dried plasma spots (DPS) | ||||
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| Abstract (PDF, 14 KB) | |||||
| Summary / Zusammenfassung | Our group has previously developed an ultrasensitive p24 antigen assay that is inexpensive, easy-to-perform, and based on use of venous plasma (Schupbach, 2003). This test does not require large and expensive equipment, which makes it suitable for smaller laboratories in resource-poor settings. Many clinical studies have been done with this assay using samples from patients with subtype B infections (Mascolini, 2002; Respess et al., 2003, 2005; Sterling et al., 2002). The assay has also been evaluated on non-B subtypes (Burgisser et al., 2000; Ribas et al., 2003) and has been introduced successfully in different African and Asian countries (de Baets et al., 2005; Sutthent et al., 2003; Stevens et al., 2005). Recently, de Baets et al. (2005) described the use of dried plasma spots (DPS) for diagnosis of pediatric infection in Central Africa and reported a 100% specificity and sensitivity for the p24 test. The goal of this project is to develop a quantitative test based on dried plasma spots to monitor HIV patients including children under antiretroviral therapy. Such a simple, low-tech, quality-assured method will allow for standard-of-care management of HIV- 1 infected patients in remote areas with no direct access to laboratories. |
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| Publications / Publikationen | Knuchel MC, Tomasik Z, Speck RF, Lüthy R, and Schüpbach J. Ultrasensitive quantitative HIV-1 p24 antigen assay adapted to dried plasma spots to improve treatment monitoring in low-resource settings. J Clin Virol 2006; 36:64–67.Weitere Informationen | ||||
| Keywords / Suchbegriffe | pediatric HIV-1 infection, resource-poor settings, DBS, HIV-1 p24 antigen, plasma HIV-1 RNA, diagnosis, viral load, DBS HIV-1 DNA PCR | ||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
Foundation PROPTER HOMINES Foundation, Vaduz, Principality of Liechtenstein |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | Mar 2005 to Jul 2006 |