Schweizer
Fakultäten » Medizinische Fakultät » Molekulare Krebsforschung, Institut für » Prof. Dr. Josef Jiricny » Schweizer
Completed research project
| Title / Titel | Microplanar synchrotron X-ray beam irradiation effects in Drosophila melanogaster | ||||
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| Abstract (PDF, 14 KB) | |||||
| Summary / Zusammenfassung | The potential of synchrotron-generated X-ray microbeams of high fluence rate in experimental and clinical radiation surgery and microbeam radiotherapy is considerable. Functional interaction or destruction of small, circumscribed targets of µm to mm dimensions may become feasible by cross-fire isocentric target using bundles of many closely spaced microbeams of subcytotoxic dose each. The few available studies on normal tissue response to microbeam irradiation revealed unusual resistance to radiation necrosis and remarkable tissue sparing effect as microbeam width is reduced from 1.0 to 0.025 mm. Moreover, persistence of the impact of sharply-defined microbeam margins in mammalian tissue seemed compromised by vasculature-related tissue microlocomotion, post irradiation hyperplasia and cell migration from adjacent areas. The purpose of this study is to display loco-regional radiopathological effects caused by microplanar synchrotron X-ray beam in tissues of pupa stage of the fruit fly Drosophila melanogaster. During this period of transient immobility, tissues undergo a precisely-timed pattern of cell differentiation in strictly mosaic-like fashion and accomplishment of morphogenesis of individual cells is didplayed in the pattern of hairs, bristles, cencillae and fascets of the epithelium of the adult. The geometrical array of the compound eye and its hexagonal retinal epithelium were considered a particularly promising tissue to monitor the spatial array of tissue microlesions . Principal radiogenic effects observed so far are 1) In the retinal epithelium the extremely sharp deliminated zone of cellular necrosis barely exeeding the beams width of 25 micrometers. 2) In cells of the wing epithelium a remarkable uniform pattern of subacute malformations within strictly limited stripes of a few cell in width proof resistence of postmitotic cells to doses as high as 3000 G..3) in surviving flies displaying no accute effects, accelerated senescence becomes evident after a latent period of weeks, when normal survival begins to decrease significantly.The possible relevance of these studies to microbeam radiation therapy are being analysed. Weitere Informationen |
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| Publications / Publikationen | Schweizer P.M., Spanne, P., Laissue J.A., Jauch U., 1997, Microplanar synchrotron x-ray beam irradiation effects in Drosophila melanogaster. Int.Conference 50th anniversery of the discovery of synchrotron radiation, Nov 17-20, 1997, ESRF, Grenoble France, Abstract Book pp. 112. | ||||
| Keywords / Suchbegriffe | X-rays, synchrotron radiation, microbeam surgery, Drosophila melanogaster | ||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
Universität Zürich (position pursuing an academic career) |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | Jan 1977 to Jan 1999 |