Hofmann-Lehmann
Fakultäten » Vetsuisse-Fakultät » Nutztiere, Departement für » Veterinärmedizinisches Labor » Prof. Dr. Regina Hofmann-Lehmann » Hofmann-Lehmann
Completed research project
| Title / Titel | Reassessment of efficacious commonly used feline leukemia virus (FeLV) vaccines by sensitive molecular methods | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | Feline leukemia virus (FeLV) is a retrovirus of great veterinary importance. In addition, FeLV infection in cats is also of human medical interest as a well-acknowledged animal model for tumor and AIDS research. For almost two decades, efficient FeLV virus vaccines have been commercially available. Sensitive molecular FeLV detection methods were developed more recently. Using these assays, we previously demonstrated that cats believed to be immune to FeLV infection turned provirus positive after virus exposure and more recently we showed that these cats remarkably also were viral plasma RNA positive. Hence, we hypothesize that also commonly used efficacious FeLV vaccines would not prevent minimal viral replication and provirus integration. Thus, it is the aim of the present project to reassess commonly used FeLV vaccines in a controlled immunization and challenge study using sensitive real-time TaqMan PCR assays to detect and quantify proviral and viral plasma RNA loads. |
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| Publications / Publikationen | Hofmann-Lehmann, R., V. Cattori, R. Tandon, F. S. Boretti, M. L. Meli, B. Riond and H. Lutz. 2008. How molecular methods change our views of FeLV infection and vaccination. Vet Immunol Immunopathol. In press.Hofmann-Lehmann, R., V. Cattori, R. Tandon, F. S. Boretti, M. L. Meli, B. Riond, A. C. Pepin, B. Willi, P. Ossent, and H. Lutz. 2007. Vaccination against the feline leukaemia virus: Outcome and response categories and long-term follow-up. Vaccine 25:5531-9.Hofmann-Lehmann, R., R. Tandon, F.S. Boretti, M.L. Meli, B. Willi, V. Cattori, M.A. Gomes-Keller, P. Ossent, M.C. Golder, J. Norman Flynn, and Hans Lutz. 2006. Reassessment of feline leukaemia virus (FeLV) vaccines with novel sensitive molecular assays. Vaccine 24: 1087-1094.Hofmann-Lehmann, R., J. B. Huder, S. Gruber, F. Boretti, B. Sigrist, and H. Lutz. 2001. Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats. J Gen Virol 82:1589-96.Hofmann-Lehmann, R., E. Holznagel, P. Ossent, and H. Lutz. 1997. Parameters of disease progression in long-term experimental feline retrovirus (feline immunodeficiency virus and feline leukemia virus) infections: hematology, clinical chemistry, and lymphocyte subsets. Clin Diagn Lab Immunol 4:33-42. | ||||||
| Keywords / Suchbegriffe | Feline leukemia virus, vaccine, cats, real-time TaqMan PCR, susceptibility, retrovirus, AIDS, animal model | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
SNF (Personen- und Projektförderung), Others SNF Professorship |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | May 2003 to Nov 2005 |