Hofmann-Lehmann
Fakultäten » Vetsuisse-Fakultät » Nutztiere, Departement für » Veterinärmedizinisches Labor » Prof. Dr. Regina Hofmann-Lehmann » Hofmann-Lehmann
Completed research project
| Title / Titel | Feline leukemia virus infection: factors that determine the susceptibility of the host | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | Feline leukemia virus (FeLV) is a gamma retrovirus that induces fatal diseases in domestic cats. The susceptibility of cats to FeLV infection varies. Besides exogenous FeLV, endogenous retrovirus-like sequences (enFeLV) are present in the genome of domestic cats and a some closely related small felids. We hypothesize that the susceptibility of individual cats may depend on the enFeLV load. Thus, the aim of this study is: - to obtain blood samples from different SPF catteries and determine enFeLV loads and in vitro infectibility with exogenous FeLV. - to analyze enFeLV loads and exogenous FeLV status in Swiss domestic cats and various wildcat populations. - to conduct controlled in vivo FeLV infection experiments with cats that have high or low enFeLV load. This project is the basis for the PhD thesis of Ravi Tandon. |
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| Publications / Publikationen | Tandon, R., V. Cattori, A.C. Pepin, B. Riond, M. McDonald, H. Lutz, and R. Hofmann-Lehmann. 2008. Association between endogenous feline leukemia virus loads and exogenous feline leukemia virus infection in domestic cats. Vir Res 135:136-43.Tandon, R., V. Cattori, B. Willi, H. Lutz, and R. Hofmann-Lehmann. 2008. Quantification of endogenous and exogenous feline leukemia virus sequences by real-time PCR assays. Vet Immunol Immunopathol 123:129-33.Cattori, V., and R. Hofmann-Lehmann. Absolute quantitation of Feline Leukemia Virus (FeLV) proviral DNA and viral RNA loads by TaqMan® real-time PCR and RT-PCR. 2008. In: Molecular beacons: Signaling nucleic acid probes, methods, and protocols. Series: Methods in Molecular Biology. Volume 429. Chapter 6. Pages 73-87. Editors: A. Marx, O. Seitz. The Humana Press Inc. Totowa, New Jersey. ISBN-10: 1588297004.Hofmann-Lehmann, R., V. Cattori, R. Tandon, F. S. Boretti, M. L. Meli, B. Riond and H. Lutz. 2008. How molecular methods change our views of FeLV infection and vaccination. Vet Immunol Immunopathol. 123:119-23.Tandon R, Cattori V, Willi B, Meli ML, Gomes-Keller MA, Lutz H, and Hofmann-Lehmann R. Copy number polymorphism of endogenous feline leukemia virus-like sequences. Molecular and Cellular Probes. Molecular & Cellular Probes 2007; 21:257-66.Hofmann-Lehmann R, Tandon R, Boretti FS, Meli ML, Willi B, Cattori V, Gomes-Keller MA, Ossent P, Golder MC, Flynn JN, and Lutz H. Reassessment of feline leukaemia virus (FeLV) vaccines with novel sensitive molecular assays. Vaccine. 2006 Feb 20;24(8):1087-94.Tandon R, Cattori V, Gomes-Keller MA, Meli ML, Golder MC, Lutz H, and Hofmann-Lehmann R. Quantitation of feline leukaemia virus viral and proviral loads by TaqMan real-time polymerase chain reaction. J Virol Methods. 2005 Dec;130(1-2):124-32.Hofmann-Lehmann, R., J. B. Huder, S. Gruber, F. Boretti, B. Sigrist, and H. Lutz. 2001. Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats. J Gen Virol 82:1589-96. | ||||||
| Keywords / Suchbegriffe | Feline leukemia virus, endogenous retrovirus, virus load | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
SNF (Personen- und Projektförderung), SNF (Programm NFP) SNF Professorship (R. Hofmann-Lehmann) |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | May 2002 to Apr 2008 |