Reichmann
Fakultäten » Medizinische Fakultät » Kinderspital: Chirurgische Klinik » TBRU » PD Dr. Ernst Reichmann » Reichmann
Completed research project
| Title / Titel | Creating long lasting blood vessels in epithelio-mesenchymal composites: An approach to efficiently vascularize tissue substitutes | ||
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| Abstract (PDF, 14 KB) | |||
| Summary / Zusammenfassung | Engineered tissue substitutes do not contain a vascular plexus that would warrant their survival and rapid integration into damaged or defected organs. Therefore the construction of stable blood vessels within the mesenchymal (stromal) component of a given tissue substitute is a fundamental challenge for tissue engineering in regenerative medicine. One focus of our work is to develop epithelio-mesenchymal tissue substitutes, e.g. novel dermo-epidermal skin grafts that have been prevascularized in vitro. We hypothesize that such a pre-vascularized graft would provide a rapid connection to the patient’s vascular system after transplantation. The rational that underlies such an approach is based on the observation that full thickness skin grafts are readily vascularized after transplantation. This is possible because the pre-existing vascular plexus of the full thickness skin can rapidly connect to the patient’s circulatory system and hence become perfused. The final or optimal pattern of the vascular network of a tissue or organ is determined by the proliferation, branching, remodeling and pruning of its different segments. The functionality and stability of blood vessels are crucially determined by the ratio of endothelial cells to their associated mural cells (pericytes, smooth muscle cells). With the above mentioned approach, we would like to contribute to the development of novel autologous epithelio-mesenchymal tissue substitutes that would mean a progress in surgical tissue substitution and hence a significant benefit for our patients. |
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| Publications / Publikationen | Pontiggia L, Biedermann T, Meuli M, Widmer D, Böttcher-Haberzeth S, Schiestl C, Schneider J, Braziulis E, Montaño I, Meuli-Simmen C, Reichmann E. Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. J Invest Dermatol. 2009 Feb;129(2):480-90.Schneider J, Biedermann T, Widmer D, Montano I, Meuli M, Reichmann E, Schiestl C. Matriderm® versus Integra®: a comparative experimental study. Burns. 2009 Feb;35(1):51-7.Montano I, Schiestl C, Schneider J, Pontiggia L, Luginbühl J, Biedermann T, Böttcher-Haberzeth S, Braziulis E, Meuli M, Reichmann E. Formation of human capillaries in vitro: The engineering of pre-vascularized matrices. Tissue Engineering Part A. 2010 Jan; 16 (1): 269-282.Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, Meuli M, and Reichmann E. Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 2010 Aug;130(8):1996-2009.Weber AD, Pontiggia L, Biedermann T, Schiestl C, Meuli M, Reichmann E. Determining the origin of cells in tissue engineered skin substitutes: a pilot study employing in situ hybridization. Pediatr Surg Int. 2010 Nov 12. [Epub ahead of print]Tharakan S, Pontiggia L, Biedermann T, Böttcher-Haberzeth S, Schiestl C, Reichmann E, Meuli M. Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells. Pediatr Surg Int. 2010 Jan; 26 (1): 71-77.Böttcher-Haberzeth S, Biedermann T, Reichmann E. Tissue Engineering of skin. Burns. 2010 Jun;36(4):450-60. Epub 2009 Dec 22. Review.Böttcher-Haberzeth S, Biedermann T, Reichmann E. Review: Wege zu einer neuen Haut: Von den zellbiologischen Grundlagen über Tissue Engineering zu einem neuen Hautsubstitut. Paediatrica. 2009; 20 (4): 57-61. |
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| Keywords / Suchbegriffe | autologous primary endothelial cells, extracellular matrix, capillaries, inosculation | ||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Other links to external web pages | http://www.skingineering.ch | ||
| Funding source(s) / Unterstützt durch |
Universität Zürich (position pursuing an academic career), Forschungskredit der Universität Zürich, EU, Other Public Sources (e.g. Federal or Cantonal Agencies), Foundation |
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| Duration of Project / Projektdauer | Mar 2003 to Dec 2011 |