Sander
Fakultäten » Medizinische Fakultät » Medizinische Mikrobiologie, Institut für » Prof. Dr. Peter Sander » Sander
Current research project
| Title / Titel | Lipoproteins: synthesis, localization and function in mycobacteria | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | Lipoprotein maturation in gram-negative bacteria is mediated by the consecutive activity of prolipoprotein diacylglycerol transferase (Lgt), lipoprotein signal peptidase (LspA), and apo-lipoprotein n-acyltransferase (Lnt). However, knowledge about mycobacterial lipoprotein transport and localisation is spare. We recently have identified lipoprotein synthesis as an important virulence factor involved in Mycobacterium tuberculosis pathogenesis. Using Mycobacterium smegmatis, a non-pathogenic, fast growing Mycobacterium as a model system, we aim to dissect mycobacterial lipoprotein synthesis and transport at the genetic, biochemical and structural level. The research project addresses 1. characterization of genes involved in the lipoprotein synthesis pathway, 2. study of defined lipoproteins, and 3. identification of lipoprotein transport mechanisms. |
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| Publications / Publikationen | Dissecting the complete lipoprotein biogenesis pahtway in Streptomyces scabies. Widdick, D.A., Hicks, M.G., Thompson, B.J., Tschumi, A., Chandra, G., Sutcliffe, I.C., Brülle, J.K., Sander, P., Palmer, T., Hutchings, M.I. (2011) Mol. Microbiol. 80: 1395 - 1412 (2011)Cloning, expression and characterization of Mycobacterium tuberculosis lipoprotein LprF. Brülle, J.K, Grau, T., Tschumi, A., Auchli, Y., Burri, R., Polsfuss, S., Keller, P.M., Hunziker, P., Sander, P. Biochem. Biophys. Res. Commun. 391: 679 – 684 (2010)Identification of apolipoprotein N-acyltransferase in mycobacteria. Tschumi, A., Nai, C., Auchli, Y., Hunziker, P., Gehrig, P., Keller, P., Grau, T., Sander, P. J. Biol. Chem. 284: 27146 – 27156 (2009)LspA inactivation in Mycobacterium tuberculosis results in attenuation without affecting phagosome maturation arrest. Rampini, S.K., Selchow, P., Keller, C., Ehlers, S., Böttger, E.C., Sander, P. Microbiology 154: 2991 - 3001 (2008)Breaking down the wall: Fractionation of mycobacteria. Rezwan, M., Laneelle, M.-A., Sander, P., Daffe, M. J. Microbiol. Methods, 68: 32-39 (2007)Lipoprotein synthesis in mycobacteria. Rezwan, M., Grau, T., Tschumi, A., Sander, P. Microbiology, 153: 653-658 (2007)Lipoprotein processing is required for virulence of Mycobacterium tuberculosis. Sander, P., Rezwan, M., Walker, B., Rampini, S.K., Kroppenstedt, R.M., Ehlers, S., Keller, C., Keeble, J.R., Hagemeier, M., Colston, M.J., Springer, B., and Böttger, E.C. Mol. Microbiol. 52: 1543 – 1552 (2004) |
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| Keywords / Suchbegriffe | Lipoprotein synthesis, protein localization, Mycobacterium, cell wall, transport, mycolic acids | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Other links to external web pages | http://www.imm.uzh.ch | ||||||
| Funding source(s) / Unterstützt durch |
Universität Zürich (position pursuing an academic career), SNF (Personen- und Projektförderung), EU |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | Mar 2003 to Sep 2016 |