Nadal
Fakultäten » Medizinische Fakultät » Kinderspital Zürich: Medizinische Klinik » Infektiologie, Abteilung » Prof. Dr. David Nadal » Nadal
Completed research project
| Title / Titel | Functional analysis of a novel, highly efficient in vitro Epstein-Barr virus infection protocol (spinoculation) compared to the conventional protocol | ||
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| Abstract (PDF, 14 KB) | |||
| Summary / Zusammenfassung | We will test the hypothesis that Epstein-Barr virus (EBV) infection in vitro by spinoculation is functionally equivalent to infection by the conventional protocol. Thus, we will use tonsillar mononuclear cells from EBV-seronegative children undergoing tonsillectomy to compare 1.) EBV entry into B-cells, 2.) B-cell subpopulations infected with EBV, and 3.) EBV gene expression in the B-cell subpopulations using spinoculation vs the conventional protocol using the EGFP-EBV and wild-type EBV. Functional equivalence of spinoculation compared to the conventional protocol will catalyze studies examining host-pathogen interactions, molecular mechanism(s) of the B-cell transformation process, and contribute to improved generation of antibodies to a plethora of antigens in vitro by EBV-transformed memory B-cells. | ||
| Keywords / Suchbegriffe | Epstein-Barr virus, in vitro infection | ||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
Foundation Hartmann-Müller |
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| Duration of Project / Projektdauer | Jan 2007 to Dec 2007 |