Böni Schüpbach
Fakultäten » Medizinische Fakultät » Medizinische Virologie, Institut für » PD Dr. Jürg Böni » Böni Schüpbach
Completed research project
| Title / Titel | Interactions of avian or porcine endogenous retroviruses with humans or human cells following enteral or parenteral exposure | ||||
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| Abstract (PDF, 14 KB) | |||||
| Summary / Zusammenfassung | Some medical procedures are associated with a parenteral exposure of humans to animal endogenous retroviruses. These include the endogenous avian retrovirus EAV-0 which is present in all chicken-cell–derived vaccines and the porcine endogenous retrovirus (PERV) contained in porcine tissues considered for xenotransplantation. We propose to investigate possible interactions of these two viruses with the human host in vitro and in vivo. The proposed investigations will address the following points: (i) the potential of these agents for infection of human cells (ii) the potential of these agents for expression in human cells (iii) the potential for interaction with co-expressed human retroviruses or members of other virus families; (iv) induction of antibody responses (v) the possibility that endogenous retroviral DNA may, as suggested by a mouse model, be taken up by the gastrointestinal tract, transported to various body tissues and lead to false-positive results in ultrasensitive molecular tests. The proposed studies address important safety questions. Retroviral safety is a central public health issue for all medical procedures that involve animal products. |
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| Publications / Publikationen | Boni J, Stalder J, Reigel F, Schupbach J. Detection of reverse transcriptase activity in live attenuated virus vaccines. Clin. Diagn. Virol. 1996; 5:43-53.Weissmahr RN, Schupbach J, Boni J. Reverse transcriptase activity in chicken embryo fibroblast culture supernatants is associated with particles containing endogenous avian retrovirus EAV-0 RNA. J. Virol. 1997; 71: 3005-3012.Shah CA, Böni J, Bisset LR, Seebach JD, Schüpbach J. Ultra-sensitive and specific detection of porcine endogenous retrovirus (PERV) using a sequence-capture real-time PCR approach. J. Virol. Methods 2003; (in press) | ||||
| Keywords / Suchbegriffe | EAV-0, PERV, vaccine, xenotransplantation, biological safety research | ||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
SNF (Personen- und Projektförderung), Other Public Sources (e.g. Federal or Cantonal Agencies) |
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| Duration of Project / Projektdauer | Oct 2000 to Dec 2004 |