Hofmann-Lehmann
Fakultäten » Vetsuisse-Fakultät » Nutztiere, Departement für » Veterinärmedizinisches Labor » Prof. Dr. Regina Hofmann-Lehmann » Hofmann-Lehmann
Completed research project
| Title / Titel | Real-time PCR investigation of the distribution and proviral and viral loads of the feline leukemia virus in tissues of cats with different infection outcome and duration | ||||||
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| Abstract (PDF, 14 KB) | |||||||
| Summary / Zusammenfassung | Feline leukemia virus (FeLV) was discovered more than 40 years ago. It is a gammaretrovirus naturally occurring worldwide in domestic cats and some related small felids. It is not only of veterinary but also of human medical interest as an animal model for tumor and AIDS research. FeLV infection can lead to fatal neoplasia, degenerative diseases of the hematopoietic system, and immunodeficiency. Most FeLV pathogenesis and vaccine studies were conducted prior to the development of sensitive, specific real-time TaqMan PCR and reverse transcriptase (RT)-PCR for the detection and quantification of FeLV proviral and viral RNA loads. It is the goal of the present study to employ these methods to further investigate FeLV pathogenesis, target tissues and influence of the viral distribution of the outcome of the infection. In particular, tissues of cats with different duration and outcome of FeLV infection will be investigated for the presence of FeLV provirus and virus and the respective loads will be quantified by real-time PCR. | ||||||
| Publications / Publikationen | Kessler Y, Helfer-Hungerbuehler AK, Cattori V, Meli ML, Zellweger B, Ossent P, Riond B, Reusch CE, Lutz H, Hofmann-Lehmann R: Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues. BMC Mol Biol 2009, 10(1):106.Hofmann-Lehmann, R., V. Cattori, R. Tandon, F. S. Boretti, M. L. Meli, B. Riond and H. Lutz. 2008. How molecular methods change our views of FeLV infection and vaccination. Vet Immunol Immunopathol. 123:119-23.Tandon, R., V. Cattori, B. Willi, H. Lutz, and R. Hofmann-Lehmann. 2008. Quantification of endogenous and exogenous feline leukemia virus sequences by real-time PCR assays. Vet Immunol Immunopathol 123:129-33.Hofmann-Lehmann, R., V. Cattori, R. Tandon, F. S. Boretti, M. L. Meli, B. Riond, A. C. Pepin, B. Willi, P. Ossent, and H. Lutz. 2007. Vaccination against the feline leukaemia virus: Outcome and response categories and long-term follow-up. Vaccine 25:5531-9.Cattori, V., R. Tandon, A. C. Pepin, H. Lutz, and R. Hofmann-Lehmann. 2006. Rapid detection of feline leukemia virus provirus integration into feline genomic DNA. Mol Cell Probes 20:172-181.Tandon, R., V. Cattori, M. A. Gomes-Keller, M. L. Meli, M. C. Golder, H. Lutz, and R. Hofmann-Lehmann. 2005. Quantitation of feline leukaemia virus viral and proviral loads by TaqMan® real-time polymerase chain reaction. J Virol Methods 130: 124-132.Hofmann-Lehmann, R., J. B. Huder, S. Gruber, F. Boretti, B. Sigrist, and H. Lutz. 2001. Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats. J Gen Virol 82:1589-96. | ||||||
| Keywords / Suchbegriffe | feline leukemia virus, retrovirus, AIDS, viral RNA load, provirus, real-time TaqMan qPCR, progressive infection, regressive infection, latency, anemia, tumor | ||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
SNF (Personen- und Projektförderung) |
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| Duration of Project / Projektdauer | Jul 2007 to Dec 2011 |