Hofmann-Lehmann
Fakultäten » Vetsuisse-Fakultät » Nutztiere, Departement für » Veterinärmedizinisches Labor » Prof. Dr. Regina Hofmann-Lehmann » Hofmann-Lehmann
Completed research project
| Title / Titel | Molecular characterization of Italian haemotrophic Mycoplasma isolates | ||||||||
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| Abstract (PDF, 14 KB) | |||||||||
| Summary / Zusammenfassung | Haemotrophic Mycoplasmas (aka haemoplasmas) are the causative agent of infectious anemia in several mammalian species. In the cat, three different haemoplasmas have been described and reported to occur in several European countries including Switzerland and the UK. In a recent study and in collaboration with our laboratory, Dr. Gentilini and his colleagues (University of Bologna) had investigated the prevalence and risk factors of hemoplasma infections in domestic cats in Bologna, Italy. Feline blood samples had been collected during a one-year period from cats presented at the Veterinary Clinical Pathology Service, Faculty of Veterinary Medicine of Bologna. Samples underwent haematological routine analyses and an aliquot of blood was stored to perform PCR assays specific for Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ and ‘Candidatus Mycoplasma turicensis’. The aim of the present study is to analyzed haemoplasma PCR-positive samples by cloning and sequencing of the 16S rRNA gene. The molecular characterization of positive sample allows conformation of the positive results of PCR assay, to molecularly characterize Italian strains of haemoplasmas and enables to identify different variants based on 16S rRNA gene. This study is conducted by Dr. Marilisa Novacco in the Clinical Laboratory, Vetsuisse Faculty, University of Zurich. |
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| Publications / Publikationen | Gentilini, F., M. Novacco, M. E. Turba, B. Willi, M. L. Bacci, R. Hofmann-Lehmann. 2009. Use of combined conventional and real-time PCR to determine the epidemiology of feline hemoplasmosis in northern Italy. J Feline Med Surg. 11(4):277-285.Willi, B., F.S. Boretti, S. Tasker, M.L. Meli, N. Wengi, C.E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2007. From Haemobartonella to hemoplasma: molecular methods provide new insights. Vet Microbiology 125:197-209.Willi, B., F. S. Boretti, C. Baumgartner, S. Tasker, B. Wenger, V. Cattori, M. L. Meli, C. E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2006. Prevalence, risk factor analysis and follow-up of infections with the three feline hemoplasma species in cats in Switzerland. J Clin Microbiol 44: 961-969.Willi, B., F. S. Boretti, V. Cattori, S. Tasker, M. L. Meli, C. E. Reusch, H. Lutz, and R. Hofmann-Lehmann. 2005. Identification, molecular characterization and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland. J Clin Microbiol 43:2581-2585. | ||||||||
| Keywords / Suchbegriffe | Haemoplasma, feline infectious anemia, haemotropic Mycoplasma, real-time PCR, 16s rRNA gene | ||||||||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Funding source(s) / Unterstützt durch |
No project-specific funding |
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| In collaboration with / In Zusammenarbeit mit |
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| Duration of Project / Projektdauer | Jan 2008 to Dec 2008 |